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Peptide synthesis services

Peptide quantification

 

Smart Bioscience offers to determine precisely the quantity of your peptides. Various methods can be used for quantifying peptides such as measurement at 280 nm in solution when applicable, weighing peptide as powder or amino acid analysis.

Weighing dried peptides: this method is not accurate because of the presence of counterions used during the purification and atmospheric water. The peptide quantity measured by weighing is named gross weight. Depending on the amino acid sequence, the net peptide content (quantity of peptidic material) can be below 50%.

Quantification by optical density measurement at 280 nm is more accurate but can only be applied on peptides containing aromatic residues (Tyr, Phe, Trp).

The gold standard method is the amino acid analysis (AAA) which gives the exact NPC (net peptide content) of a sample. Smart Bioscience offers to perform AAA analysis for determining the exact quantity of its client’s peptides.

Contact us to discuss about your project.